Head of the Virology Laboratory is Vladimir Vladimirovich Kirpichenko, Candidate of Veterinary Sciences.

Laboratory of Virology was organized in 2012 by combining the foot-and-mouth disease laboratory (1936), the Laboratory of Avian diseases (1961) and the Laboratory of Virology (1959).

The first head and founder of the Virology Laboratory was Candidate of Veterinary Sciences A.A. Palgov, then it was headed by Doctor of Veterinary Sciences, Professor K.N. Buchnev and Doctor of Veterinary Sciences G.A. Kolomakin.

From 1974 to 1995, the laboratory was headed by Doctor of Veterinary Sciences, Professor N.N. Gizitdinov; in 1995-1998 – Candidate of Biological Sciences J.O. Omirzhanov; in 1998-2000 – Candidate of Veterinary Sciences L.B. Kutumbetov; in 2000-2004 – Candidate of Veterinary Sciences Y.H. Bakhtakhunov; in 2004-2012 – Candidate of Veterinary Sciences A.A. Abishov; in 2012-2018 – Doctor of Veterinary Sciences, Associate Professor L.B. Kutumbetov; in 2019-2022 – Master of Agricultural Sciences M.A. Saduakasova; since 2023, the virology laboratory is headed by Candidate of Veterinary Sciences V.V. Kirpichenko.

Research directions and objectives:

Disease diagnostics: Detection and identification of pathogens using PCR (polymerase chain reaction), ELISA and virus isolation on virological cultures.

Disease monitoring: Active information monitoring of the spread of infectious diseases among animals inside and outside the country and providing data on disease incidence.

Research and development: The Laboratory of Virology is engaged in scientific research aimed at developing new diagnostic methods, national standards, vaccines, strategies and methods for the control of animal infectious diseases.

Training and consultations: The Virology Laboratory provides training programs for veterinary specialists, undergraduate and graduate students and advises practicing veterinarians and economic entities on the diagnosis and control of infectious diseases.

Analysis and forecasting: The laboratory participates in epizootological monitoring and data analysis, which allows timely detection and prevention of disease outbreaks, and predicting their possible development.

Main achievements

During the period of the laboratory’s existence, its employees have developed and implemented:

– a manufacturing method of anti-foot-and-mouth disease serum and citrated blood of convalescents, which was included in the instruction on FMD control;

– a method for diagnosing rabies using precipitation reaction in agarose gel;

– homologous anti-rabies vaccine against canine rabies;

– preventive measures and treatment of viral respiratory and gastrointestinal diseases of young cattle;

– polyalent immunolactoserum against calf pneumoenteritis, which has 85-90% preventive and curative effect;

– vaccine manufacture technologies against equine herpesvirus infection, rabbit viral hemorrhagic disease, sheep and goat pox;

– 12 methodological recommendations aimed at combating and preventing infectious diseases of animals, including especially dangerous ones;

– Strategy for the control of foot-and-mouth disease in animals;

– Self-declaration on avian influenza;

– panel of standard national serums for bovine leukosis;

– diagnostic test system for detection of avian influenza virus in RT-PCR.

The Virology Laboratory has permission to handle pathogens of II-IV group pathogenicity, accreditation area according to ISO/IEC – 17025-2019 standards, is a provider of proficiency testing of diagnostic laboratories and organizes rounds of comparison tests both at national and international levels.

The laboratory conducts research (scope of accreditation):

RNA detection of avian influenza virus type A by real-time RT-PCR;

RNA detection of avian influenza virus subtypes H5 and H7 by real-time RT-PCR;

RNA detection of Newcastle disease virus by real-time RT-PCR;

detection of antibodies to avian influenza virus by enzyme immunoassay;

detection of antibodies to Newcastle disease virus by hemagglutination inhibition reaction;

detection of antibodies to avian influenza virus by hemagglutination inhibition reaction;

detection of antibodies to Newcastle disease virus by immunoassay method;

detection of antibodies to bovine leukosis virus by immunoassay method;

detection of antibodies to lumpy skin disease virus by immunoassay method;

detection of antibodies to avian infectious laryngotracheitis virus;

detection of antibodies to avian infectious bronchitis virus.

Scientific relations have been established with the Veterinary Research Institute “OIE National Reference Laboratory for Bovine Leukosis” (Piwet, Poland), OIE / FAO FMD Pirbright Institute (UK), FGBI “ARRIAH” (Russia).

Scientific publications:

1. Turgenbaev K.A, Abdybekova, A.M, Borsynbayeva, Kirpichenko V.V, Aiken S. Karabassova, Yerzhan Ospanov Saltanat Mamanova Perizat Akshalova Elvira Bashenova Saira Kaymoldina Marat Turkeev Bakhit Tulepov. Development and planning of measures to reduce the risk of the foot-and-mouth disease virus spread (case of the Republic of Kazakhstan). // Caspian journal of Environmental Sciences, ISSN 1735-3033. – July 2023. – Volume 21, Issue 3. – P. 561-573. doi.10.22124/CJES.2023.6933.
2. Aneta Pluta, Tasia Marie Taxis, Frank van der Meer, Sulav Shrestha, Dominic Qualley, Paul Coussens, Marzena Rola-Łuszczak, Anna Ryło, Ali Sakhawat, Saltanat Mamanova, and Jacek Kuźmak. An immunoinformatics study reveals a new BoLA-DR-restricted CD4+ T cell epitopes on the Gag protein of bovine leukemia virus. // Sci Rep 13, 22356 (2023). https://doi.org/10.1038/s41598-023-48899-4. (Q1 в WoS, процентиль 78)
3. Kirpichenko V.V., Bakieva F.A., Mamanova S.B., Ospanov E.K., Kajmoldina S.E. Epizooticheskaya situaciya po infekcionnomu rinotraheitu krupnogo rogatogo skota v Karagandinskoj oblasti, Respublika Kazahstan, v 2021-2022 godah // Veterinariya segodnya. – 2023-09 DOI: 10.29326/2304-196X-2023-12-3-233-239.
4. Ospanov Y.K., Kanatbayev S.G., Akshalova P.B., Mamanova S.B., Bashenova E.E., Karabassova A.S., Kaymoldina S.Y. Risks of occurrence and spread of lumpy skin disease virus in the republic of kazakhstan // Nauchno-prakticheskij zhurnal Zapadno-Kazahstanskogo agrarno-tekhnicheskogo universiteta imeni ZHangir hana «Ғylym zhəne bіlіm». – 2023. – №1-1/70. – S. 97-105. https://doi.org/10.52578/2305-9397-2023-1-1-97-105.
5. Karabasova A.S., Turgenbaev K.A., Ospanov E.K., Bashenova E.E., Mamanova S.B., Alihanov K.D., ZHusupbekov ZH.S., Turkeev M.K., Borsynbaeva A.M. Immunogennost’ protivoyashchurnyh vakcin primenyaemyh v Kazahstane. // Nauchno-prakticheskij zhurnal Zapadno-Kazahstanskogo agrarno-tekhnicheskogo universiteta imeni ZHangir hana, «Gylym zhane bіlіm». – 2023. – T. 2. – №2-2(71). S. 39-47.
6. Kirpichenko V.V., Bakieva F.A., Mamanova S.B., Ospanov E.K., Kajmoldina S.E. Epizooticheskaya obstanovka po infekcionnomu rinotraheitu krupnogo rogatogo skota v Karagandinskoj oblasti Respubliki Kazahstan v 2021–2022 gg. // Veterinariya segodnya. FGBU «Federal’nyj centr ohrany zdorov’ya zhivotnyh». Moskva, 2023; – 12(3): – S. 233–239. DOI: 10.29326/2304-196X-2023-12-3-233-239.
7. Bashenova E.E., Nisanova R.K., ZHusupbekov ZH.S., Begasyl K.S., Saduakasova M.A., Қarabasova A.S., Alihanov Қ.D. «Құs tұmauy virusyna antidenelerdі anyқtau үshіn serologiyalyқ әdіsterdі keshendі pajdalanu zhәne standartty қan sarysuyna үlgіlerdі taңdau» // v Nauch.- prakt. ZHurnal ZKATU imeni ZHangir hana «Gylym zhane bіlіm» – 2023. – T. 3. – № 2-1 (71). – S.29-36.
8. Akhmetzhan Sultanov, Marzena Rola-Łuszczak, Saltanat Mamanovа, Anna Ryło, Zbigniew Osi ́nski, Meruyert A. Saduakassova, Elvira Bashenova and Jacek Ku ́zmak. Molecular Characterization of Bovine Leukemia Virus with the Evidence of a New Genotype Circulating in Cattle from Kazakhstan // Journal Pathogens. – 2022. – Р 11(2):180. (https://doi.org/10.3390/pathogens11020180).
9. Nesterov, A.; Mazloum, A.; Byadovskaya, O.; Shumilova, I.; Van Schalkwyk, A.; Krotova, A.; Kirpichenko, V.; Donnik, I.; Chvala, I.; Sprygin, A. Experimentally controlled study indicates that the naturally occurring recombinant vaccine-like lumpy skin disease strain Udmurtiya/2019, detected during freezing winter in northern latitudes, is transmitted via indirect contact // Frontiers in Veterinary Science. – 2022. DOI: 10.3389/fvets.2022.1001426.
10. Aiken S. Karabassova, Akhmetzhan A. Sultanov, Meruyert A. Saduakassova, Donald P. King, Anna B. Ludi, Clare F. J. Browning and Ginette Wilsden. Toward the calibration of serological assays using sera collected from cattle and sheep following a single dose of foot-and-mouth disease vaccine. // Veterinary World. – 2022. – V. 15. – P. 524-530. www.doi.org/10.14202/vetworld.2022.524-530.
11. Zinyakov, N.G., Sosipatorova, V.Y., Andriyasov, A.V., Akshalova, P.B. [et al.] Genetic analysis of genotype G57 H9N2 avian influenza virus isolate A/chicken/Tajikistan/2379/2018 recovered in Central Asia // Arch Virol. – 2021. – N 166 (6). – P. 1591–1597. DOI: 10.1007/s00705-021-05011-3.
12. P.B. Akshalova, A.V. Andriyasov, L.O. SHCHerbakova [i dr.] Razrabotka metoda OT-PCR v rezhime real’nogo vremeni dlya vyyavleniya RNK virusa grippa ptic podtipa N2 // Veterinariya segodnya. – 2020. – №3 (34). – S. 186–192. DOI: 10.29326/2304- 196X-2020-3-34-186-192.
13. Patent «Sposob polucheniya syvorotki dlya provedeniya diagnosticheskih issledovanij pri bolezni N’yukasla». Patent №7287 (21) 2022/0394.2. avt. ZHusupbekov ZH.S., Saduakasova M.A., Bajkara B.
14. Patent «Sposob polucheniya syvorotki dlya provedeniya diagnosticheskih issledovanij pri grippe ptic». Patent №7496 (21) 2022/0393.2. avt. ZHusupbekov ZH.S., Saduakasova M.A., Bajkara B.

e-mail: vlad_92reik@mail.ru